1LZN

NEUTRON STRUCTURE OF HEN EGG-WHITE LYSOZYME

Experimental procedure

Experimental method	LAUE
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1997-12
Wavelength(s)	2.7-3.5
Spacegroup name	P 1
Unit cell lengths	27.280, 32.040, 34.270
Unit cell angles	88.80, 108.80, 111.60

Refinement procedure

Resolution	13.600 - 1.700
R-factor	0.204 *
Rwork	0.204
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMEN XPLOR
Starting model (for MR)	4lzt
RMSD bond length	0.016
RMSD bond angle	0.033
Data reduction software	CCP4 (LAUE SUITE)
Data scaling software	CCP4 ((LAUE SUITE))
Refinement software	CCP4 (LAUE SUITE MODIFIED FOR CYLINDER GEOMETRY (C. WILKINSON))

Data quality characteristics

	Overall
Low resolution limit [Å]	13.600
High resolution limit [Å]	1.700
Rmerge	0.146
Total number of observations	46061 *
Number of reflections	8976
<I/σ(I)>	35
Completeness [%]	83.0
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	4.7		BATCH TECHNIQUE EQUAL AMOUNT OF 1.0 % SOLUTION OF HEN EGG-WHITE LYSOZYME ( BOEHRINGER) AND 2.8 % NANO3 IN 50 MM ACETATE BUFFER (PH 4.7)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	1.0 (%)
2	1	1		2.8 (%)
3	1	1	acetate	50 (mM)