1LZ8

LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1994-12-01
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	78.810, 78.810, 36.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.530
R-factor	0.22
Rwork	0.220
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8lyz
RMSD bond length	0.026
RMSD bond angle	0.044
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000 *	0.940
High resolution limit [Å]	1.530 *	1.530 *
Rmerge	0.046 *	0.190 *
Number of reflections	58373
<I/σ(I)>	29.1	4.9
Completeness [%]	100.0 *	100 *
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	4.6		BATCH METHOD. 20 MG/ML PROTEIN SOLUTION IN 50MM SODIUM ACETATE PH 4.6 AND 10% NACL. CRYSTALS GROWN AT ROOM TEMPERATURE.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1		10 (%(w/v))