1LYX
Plasmodium Falciparum Triosephosphate Isomerase (PfTIM)-Phosphoglycolate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.242, 63.437, 53.886 |
| Unit cell angles | 90.00, 117.74, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.180 |
| R-free | 0.21100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Monomer of unbound PfTIM PDB code 1YDV |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.352 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 * | |
| Total number of observations | 198026 * | |
| Number of reflections | 10508 * | |
| Completeness [%] | 98.4 | 97.1 |
| Redundancy | 18.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 12% to 25% PEG 6000 or PEG 4000 or PEG 3350 or PEG 1450 in the presence of EDTA, DTT and Sodium azide in 100mM MES. pH 6.5, VAPOR DIFFUSION, HANGING DROP at 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 12-25 (%) | |
| 2 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
| 3 | 1 | reservoir | dithiothreitol | 0.05 (mM) | |
| 4 | 1 | reservoir | EDTA | 0.05 (mM) | |
| 5 | 1 | reservoir | 0.05 (mM) | ||
| 6 | 1 | drop | protein | 15 (mg/ml) |
