1LYQ
Crystal Structure of PcoC, a Methionine Rich Copper Resistance Protein from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 81 |
| Detector technology | CCD |
| Collection date | 2000-01-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9700 |
| Spacegroup name | P 41 |
| Unit cell lengths | 70.123, 70.123, 39.072 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb from MAD solution |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.086 | 0.263 |
| Number of reflections | 29303 | |
| <I/σ(I)> | 6.2 | 2.3 |
| Completeness [%] | 95.6 | 96.1 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
