1LYL
LYSYL-TRNA SYNTHETASE (LYSU) (E.C.6.1.1.6) COMPLEXED WITH LYSINE
Experimental procedure
| Collection date | 1993-08-25 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 144.270, 257.800, 182.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.800 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.23800 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.320 |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.078 * |
| Number of reflections | 76222 |
| Completeness [%] | 92.8 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
| 4 | 1 | drop | lysine | 5 (mM) | |
| 5 | 1 | reservoir | PIPES | 0.1 (M) | |
| 6 | 1 | reservoir | PEG2000 | 20 (%(w/v)) | |
| 7 | 1 | reservoir | 0.5 (M) |
