1LY9
The impact of the physical and chemical environment on the molecular structure of Coprinus cinereus peroxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04-18 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.600, 116.940, 36.800 |
Unit cell angles | 90.00, 90.70, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.000 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 22.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 * | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.071 | 0.210 |
Total number of observations | 73087 * | |
Number of reflections | 40199 | |
Completeness [%] | 90.9 | 74.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.25 | 293 | 1.8M NH4H2PO4, pH 4.00-4.50, VAPOR DIFFUSION, HANGING DROP at 293K, pH 4.25 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 1.8 (M) | ||
2 | 1 | drop | protein | 17 (mg/ml) |