1LXK
Streptococcus pneumoniae Hyaluronate Lyase in Complex with Tetrasaccharide Hyaluronan Substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2001-03-20 |
| Detector | OXFORD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.736, 103.817, 101.347 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.520* |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.235 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.520 * | 1.520 * |
| Rmerge | 0.080 * | 0.611 * |
| Total number of observations | 904361 * | |
| Number of reflections | 124927 * | |
| Completeness [%] | 93.1 | 49.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 22 * | AMMONIUM SULFATE, SODIUM Cacodylate, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.4 |
| 3 | 1 | drop | EDTA | 2 (mM) | |
| 4 | 1 | drop | DL-dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.0 |
| 6 | 1 | reservoir | ammonium sulfate | 2.9 (M) | |
| 7 | 1 | reservoir | EDTA | 5 (mM) |
