1LXD
CRYSTAL STRUCTURE OF THE RAS INTERACTING DOMAIN OF RALGDS, A GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR OF RAL PROTEIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-10 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.282, 30.714, 51.326 |
| Unit cell angles | 90.00, 94.57, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.400 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.29800 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MADSYS |
| Refinement software | X-PLOR (3.85) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 * | |
| Number of reflections | 6485 | |
| <I/σ(I)> | 16.7 | 5.92 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | PROTEIN WAS CRYSTALLIZED FROM 20% PEG 8000, 0.1 M TRIS PH 8.5, AND 0.2 M CALCIUM ACETATE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | PEG8000 | 20 (%) | |
| 2 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 3 | 1 | drop | PMSF | 0.5 (mM) | |
| 4 | 1 | drop | EDTA | 0.5 (mM) | |
| 5 | 1 | drop | calcium acetate | 0.1 (M) | |
| 6 | 1 | drop | Tris-HCl | 0.05 (mM) | |
| 7 | 1 | drop | PEG8000 | 10 (%) | |
| 8 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 9 | 1 | reservoir | Tris-HCl | 0.1 (M) |
