1LWI

3-ALPHA-HYDROXYSTEROID/DIHYDRODIOL DEHYDROGENASE FROM RATTUS NORVEGICUS

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	46.900, 139.100, 53.300
Unit cell angles	90.00, 113.50, 90.00

Refinement procedure

Resolution	5.000 - 2.700
R-factor	0.234
Rwork	0.234
R-free	0.29400
RMSD bond length	0.012
RMSD bond angle	25.200 *
Data reduction software	MOSFLM
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.700
Rmerge	0.058
Total number of observations	53539 *
Number of reflections	16123
Completeness [%]	90.0
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	binary complex	10 (mg/ml)
2	1	reservoir	PEG4000	27 (%(w/v))
3	1	reservoir	ammonium acetate	0.19 (M)
4	1	reservoir	sodium acetate	0.10 (M)
5	1	reservoir	dimethyl sulfoxide	1.3 (%(v/v))