1LWB

Crystal structure of prokaryotic phospholipase A2 at atomic resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	297
Detector technology	IMAGE PLATE
Collection date	1997
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.71073
Spacegroup name	P 1 21 1
Unit cell lengths	29.340, 57.495, 31.807
Unit cell angles	90.00, 111.07, 90.00

Refinement procedure

Resolution	10.000 - 1.050
R-factor	0.103 *
Rwork	0.103
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1faz
RMSD bond length	0.022
RMSD bond angle	2.500 *
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	0.954
High resolution limit [Å]	0.923	0.923
Rmerge	0.072	0.228
Number of reflections	45532	1418 *
<I/σ(I)>	8.21	1.64
Completeness [%]	67.5	22.75
Redundancy	2.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	297	Matoba, Y., (2002) J.Biol.Chem., 277, 20059. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)
2	1	drop		20 (mM)
3	1	drop	protein	20 (mg/ml)
4	1	reservoir	PEG6000	14 (%(w/v))
5	1	reservoir		0.2 (M)
6	1	reservoir	sodium cacodylate	0.1 (M)