1LW9
Multiple methionine substitutions are tolerated in T4 lysozyme and have coupled effects on folding and stability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-14 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.207, 60.207, 95.375 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.000 - 1.450 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.641 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.100 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.075 | 0.316 |
| Number of reflections | 35919 | |
| <I/σ(I)> | 4.8 | 2.1 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 5.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | pH 6.7 |
