1LVL

THE REFINED STRUCTURE OF PSEUDOMONAS PUTIDA LIPOAMIDE DEHYDROGENASE COMPLEXED WITH NAD+ AT 2.45 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	64.320, 108.120, 151.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.450
R-factor	0.215
RMSD bond length	0.022 *
RMSD bond angle	4.000 *
Refinement software	GROMOS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000 *
High resolution limit [Å]	2.450 *	2.450 *
Rmerge	0.072 *	0.189 *
Number of reflections	18731 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	NAD+	20 (mM)
3	1	drop	PEG6000	16 (%(w/v))
4	1	drop	HEPES	50 (mM)
5	1	drop	EDTA	0.5 (mM)
6	1	drop		0.02 (%)
7	1	reservoir	PEG6000	15 (%)