1LVK
X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 174 |
| Detector technology | CCD |
| Collection date | 1997-04 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 103.900, 179.900, 53.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.2 * |
| Rwork | 0.200 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 16.880 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.085 | 0.168 |
| Total number of observations | 376628 * | |
| Number of reflections | 79437 | |
| <I/σ(I)> | 58.5 | 20.3 |
| Completeness [%] | 98.6 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | mantATP | 2 (mM) | |
| 2 | 1 | 1 | 15 (mM) | ||
| 3 | 1 | 1 | 3 (mM) | ||
| 4 | 1 | 1 | 3 (mM) | ||
| 5 | 1 | 2 | PEG8000 | 7.9 (%(w/v)) | |
| 6 | 1 | 2 | HEPES | 50 (mM) | |
| 7 | 1 | 2 | 120 (mM) | ||
| 8 | 1 | 2 | dithiothreitol | 3 (mM) | |
| 9 | 1 | 2 | ethylene glycol | 2 (%(w/v)) |
