1LUZ

Crystal Structure of the K3L Protein From Vaccinia Virus (Wisconsin Strain)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	200
Detector technology	CCD
Collection date	2000-05-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9788,0.9790,1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	73.119, 49.580, 59.938
Unit cell angles	90.00, 100.69, 90.00

Refinement procedure

Resolution	500.000 - 1.800
Rwork	0.232
R-free	0.25600
Structure solution method	MAD
RMSD bond length	0.005 *
RMSD bond angle	1.290 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	500.000
High resolution limit [Å]	1.800
Rmerge	0.079 *
Total number of observations	170730 *
Number of reflections	12629 *
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1		300 (mM)
3	1	1	sodium phosphate	30 (mM)	pH7.0
4	1	1	dithiothreitol	5 (mM)