1LUQ
Full Matrix Error Analysis of Streptavidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.027, 93.540, 104.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.000 - 0.960 |
| Rwork | 0.139 |
| R-free | 0.16450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1df8 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.036 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 0.970 |
| High resolution limit [Å] | 0.960 | 0.960 |
| Rmerge | 0.065 | 0.602 |
| Number of reflections | 135985 | |
| Completeness [%] | 96.4 | 45 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | ammonium sulfate, sodium acetate, biotin, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
