1LUE
RECOMBINANT SPERM WHALE MYOGLOBIN H64D/V68A/D122N MUTANT (MET)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-11-07 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 91.200, 91.200, 45.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.120 - 1.700 |
| Rwork | 0.168 |
| R-free | 0.20200 |
| Structure solution method | Isomorphic with wild-type |
| RMSD bond length | 0.011 |
| RMSD bond angle | 17.100 * |
| Data reduction software | XDS |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 1.810 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.088 | |
| Number of reflections | 22467 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 93.8 | 73.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | ammonium sulfate, tris-HCL, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 3.2 (M) | |
| 2 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | EDTA | 1 (mM) | pH9.0 |
| 4 | 1 | reservoir | ammonium sulfate | 2.4-2.6 (M) |
