1LU1
THE STRUCTURE OF THE DOLICHOS BIFLORUS SEED LECTIN IN COMPLEX WITH THE FORSSMAN DISACCHARIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 79.050, 79.050, 260.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fat |
| RMSD bond length | 0.080 * |
| RMSD bond angle | 1.460 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.620 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.146 * | 0.750 |
| Total number of observations | 91892 * | |
| Number of reflections | 13175 | |
| <I/σ(I)> | 15.2 | 2.27 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 100 MM HEPES PH 7.5 0.2 M MGCL2 30% (W/V) PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | adenine | 5 (mM) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | PEG400 | 30 (%(w/v)) |
