1LTL

THE DODECAMER STRUCTURE OF MCM FROM ARCHAEAL M. THERMOAUTOTROPHICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-08
Detector	SBC-2
Wavelength(s)	1.03320
Spacegroup name	H 3 2
Unit cell lengths	192.321, 192.321, 365.539
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 * - 3.000
Rwork	0.245
R-free	0.29500
Structure solution method	SIR + SAD
RMSD bond length	0.021 *
RMSD bond angle	4.207 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	3.000
Rmerge	99.900 *	0.470 *
Total number of observations	835966 *
Number of reflections	56524 *
Completeness [%]		100 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	18 *	Hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	50 (mM)	pH8.0
2	1	reservoir	sodium citrate	0.8 (M)
3	1	reservoir	glycerol	10 (%(v/v))
4	1	drop	protein	20 (mg/ml)