1LTE

STRUCTURE OF A LEGUME LECTIN WITH AN ORDERED N-LINKED CARBOHYDRATE IN COMPLEX WITH LACTOSE

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	84.400, 73.050, 71.400
Unit cell angles	90.00, 113.42, 90.00

Refinement procedure

Resolution	? - 2.000
R-factor	0.19
Rwork	0.190
RMSD bond length	0.018
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.052 *
Total number of observations	123808 *
Number of reflections	26597 *
Completeness [%]	99.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	lactose	10 (mM)
3	1	drop	MPD	15 (%)
4	1	drop	sodium azide	0.01 (%)
5	1	drop	HEPES	50 (mM)
6	1	reservoir	MPD	30 (%)
7	1	reservoir	HEPES	100 (mM)