1LTA

2.2 ANGSTROMS CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN (LT) WITH BOUND GALACTOSE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	70.700, 73.500, 163.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.200
R-factor	0.175
Rwork	0.175
RMSD bond length	0.015
RMSD bond angle	3.020
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.049 *
Total number of observations	85016 *
Number of reflections	38700 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	three-layer liquid-liquid diffusion *	7.5 *		all solutions contain TEA buffer *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15 (mg/ml)
2	1	1	Tris-HCl	100 (mM)	TEA buffer
3	1	1	EDTA	1 (mM)	TEA buffer
4	1	1	sodium azide	0.02 (%)	TEA buffer
5	1	1		0.2 (M)
6	1	2	galactose	50 (mM)
7	1	3	PEG6000	40 (%)