1LT5
HEAT-LABILE ENTEROTOXIN B-PENTAMER COMPLEXED WITH THIODIGALACTOSIDE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.680, 100.130, 63.670 |
| Unit cell angles | 90.00, 107.66, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lta |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.065 | 0.268 |
| Number of reflections | 43351 | |
| <I/σ(I)> | 13 | 3.5 |
| Completeness [%] | 77.0 | 81 |
| Redundancy | 2.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 | three-layered solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | ||
| 10 | 1 | 3 | 50 (mM) | ||
| 2 | 1 | 1 | Tris-HCl | 100 (mM) | |
| 3 | 1 | 1 | 200 (mM) | ||
| 4 | 1 | 1 | EDTA | 0.5 (mM) | |
| 5 | 1 | 1 | azide | 0.1 (mM) | |
| 6 | 1 | 2 | thiodigalactoside | 200 (mM) | |
| 7 | 1 | 2 | Tris-HCl | 100 (mM) | |
| 8 | 1 | 3 | PEG6000 | 42 (%) | |
| 9 | 1 | 3 | Tris-HCl | 100 (mM) |
