1LT4
HEAT-LABILE ENTEROTOXIN MUTANT S63K
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 120.000, 101.900, 64.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.25500 |
| Structure solution method | ISOMORPHOUS MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ltt |
| RMSD bond length | 0.011 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.063 * | 0.156 * |
| Number of reflections | 50516 | |
| <I/σ(I)> | 18 | 5.1 |
| Completeness [%] | 93.3 | 81.4 * |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | three-layer capillary * | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 5% PEG 6000, 100 MM NACL, 1 MM EDTA, 75 MM LACTOSE, 100 MM TRIS PH 7.5 USING THE 3 LAYER CAPPILARY METHOD, 3 layer capillary method |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | LT-I Ser63Lys | 5 (mg/ml) | |
| 2 | 1 | 1 | PEG6000 | 5.6 (%) | |
| 3 | 1 | 1 | Tris | 100 (mM) | |
| 4 | 1 | 1 | 100 (mM) | ||
| 5 | 1 | 1 | 0.02 (%) | ||
| 6 | 1 | 1 | lactose | 75 (mM) |
