1LST

THREE-DIMENSIONAL STRUCTURES OF THE PERIPLASMIC LYSINE-, ARGININE-, ORNITHINE-BINDING PROTEIN WITH AND WITHOUT A LIGAND

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	37.790, 59.630, 115.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.165
Rwork	0.165
RMSD bond length	0.013
RMSD bond angle	2.540
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.041 *
Total number of observations	92644 *
Number of reflections	23359 *
Completeness [%]	93.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	sodium acetate	0.1 (M)	precipitant
3	1	drop	sodium cacodylate	0.05 (M)	precipitant
4	1	drop	PEG4000	25 (%)	precipitant
5	1	reservoir	precipitant		20 ml