1LST
THREE-DIMENSIONAL STRUCTURES OF THE PERIPLASMIC LYSINE-, ARGININE-, ORNITHINE-BINDING PROTEIN WITH AND WITHOUT A LIGAND
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.790, 59.630, 115.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.800 |
R-factor | 0.165 |
Rwork | 0.165 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.540 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.800 * |
Rmerge | 0.041 * |
Total number of observations | 92644 * |
Number of reflections | 23359 * |
Completeness [%] | 93.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | sodium acetate | 0.1 (M) | precipitant |
3 | 1 | drop | sodium cacodylate | 0.05 (M) | precipitant |
4 | 1 | drop | PEG4000 | 25 (%) | precipitant |
5 | 1 | reservoir | precipitant | 20 ml |