1LSS
KTN Mja218 CRYSTAL STRUCTURE IN COMPLEX WITH NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-11 |
| Detector | SBC-2 |
| Wavelength(s) | 1.0451 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.000, 74.400, 86.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.300 |
| Rwork | 0.220 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.208 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 * | 0.287 |
| Number of reflections | 23827 | |
| <I/σ(I)> | 14.9 | 3.7 |
| Completeness [%] | 92.0 * | 72.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | Magnesium Formate, Ammonium Citrate, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6-8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | NAD(H) | 1 (mM) | |
| 5 | 1 | reservoir | magnesium formate | 200 (mM) | |
| 6 | 1 | reservoir | ammonium citrate | 75 (mM) | |
| 7 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
