1LSH
LIPID-PROTEIN INTERACTIONS IN LIPOVITELLIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-02-07 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.170, 84.520, 89.530 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.25500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | LIPOVITELLIN (ROOM TEMP) |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 * | |
| Number of reflections | 82007 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 74.4 | 30.1 |
| Redundancy | 1.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 18 * | Protein stock:10 mg/ml lipovitellin, 0.25 mM sodium citrate; Well: 0.55-0.61 M sodium citrate, 1mM thioglycerol, 1 mM EDTA, 0.05% sodium azide, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 0.25 (M) | pH6.2 |
| 3 | 1 | reservoir | sodium citrate | 0.55-0.61 (M) | pH6.2 |
| 4 | 1 | reservoir | thioglycerol | 1 (mM) | |
| 5 | 1 | reservoir | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | sodium azide | 0.05 (%) |
