1LSH

LIPID-PROTEIN INTERACTIONS IN LIPOVITELLIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1998-02-07
Detector	CUSTOM-MADE
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	190.170, 84.520, 89.530
Unit cell angles	90.00, 100.39, 90.00

Refinement procedure

Resolution	30.000 * - 1.900
R-factor	0.193
Rwork	0.193
R-free	0.25500
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	LIPOVITELLIN (ROOM TEMP)
RMSD bond length	0.019
RMSD bond angle	2.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.990
High resolution limit [Å]	1.900	1.900
Rmerge	0.064 *
Number of reflections	82007
<I/σ(I)>	12.2
Completeness [%]	74.4	30.1
Redundancy	1.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	18 *	Protein stock:10 mg/ml lipovitellin, 0.25 mM sodium citrate; Well: 0.55-0.61 M sodium citrate, 1mM thioglycerol, 1 mM EDTA, 0.05% sodium azide, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium citrate	0.25 (M)	pH6.2
3	1	reservoir	sodium citrate	0.55-0.61 (M)	pH6.2
4	1	reservoir	thioglycerol	1 (mM)
5	1	reservoir	EDTA	1 (mM)
6	1	reservoir	sodium azide	0.05 (%)