1LRZ
x-ray crystal structure of staphylococcus aureus femA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.96863, 0.97949, 0.97923 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.930, 90.360, 109.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.21200 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.350 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNX (2000.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 * | 0.246 * |
| Total number of observations | 236796 * | |
| Number of reflections | 32411 * | |
| <I/σ(I)> | 22.3 | 3 |
| Completeness [%] | 99.7 * | 97.9 |
| Redundancy | 7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 10 * | 4 * | PEG 8000, calcium acetate, imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 2 | ethanolamine | 50 (mM) | |
| 2 | 1 | 2 | dithiothreitol | 1 (mM) | pH10.0 |
| 3 | 1 | 1 | protein | 12 (mg/ml) |
