1LRV
A LEUCINE-RICH REPEAT VARIANT WITH A NOVEL REPETITIVE PROTEIN STRUCTURAL MOTIF
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.800, 69.800, 159.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.27200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 25.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.077 | 0.288 |
| Total number of observations | 63817 * | |
| Number of reflections | 13656 | |
| <I/σ(I)> | 14.3 | 3 |
| Completeness [%] | 94.0 | 92.4 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8.5 | THE PROTEIN WAS CRYSTALLIZED BY THE MICROCAPILLARY BATCH DIFFUSION METHOD IN 1.2 M AMMONIUM SULFATE, 1 MM SODIUM DITHIONITE BUFFERED WITH TRIS-HCL PH 8.5, microcapillary batch diffusion |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 2.0 (M) | |
| 2 | 1 | 1 | sodium dithionite | 1 (mM) | |
| 3 | 1 | 1 | Tris-HCl | pH8.5 |
