1LRA
CRYSTALLOGRAPHIC STUDY OF GLU 58 ALA RNASE T1(ASTERISK)2'-GUANOSINE MONOPHOSPHATE AT 1.9 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.440, 49.640, 26.090 |
| Unit cell angles | 90.00, 99.75, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.178 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.059 |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 * |
| Rmerge | 0.069 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4.2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 1.5 (%(w/v)) | |
| 2 | 1 | drop | 2'-GMP | 0.125 (%) | |
| 3 | 1 | reservoir | MPD | 55 (%(v/v)) | |
| 4 | 1 | reservoir | sodium acetate | 20 (mM) | |
| 5 | 1 | reservoir | calcium acetate | 2 (mM) |
