1LR4

Room Temperature Crystal Structure of the Apo-form of the catalytic subunit of protein kinase CK2 from Zea mays

Replaces:  1A6O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-10-02
Detector	MARRESEARCH
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	142.550, 61.440, 45.410
Unit cell angles	90.00, 103.06, 90.00

Refinement procedure

Resolution	26.000 - 2.000
R-factor	0.193 *
Rwork	0.193
R-free	0.23700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	BACKBONE OF CYCLIN-DEPENDENT KINASE 2 (PDB entry 1HCL)
RMSD bond length	0.016
RMSD bond angle	1.520
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000 *	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.106 *	0.111 *
Number of reflections	24342
Completeness [%]	93.7 *	84.9
Redundancy	2.8	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.1 *	20 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	20 (%(w/v))
2	1	reservoir	sodium acetate	200 (mM)
3	1	reservoir	Tris-HCl	100 (mM)	pH8.1