1LQ9

Crystal Structure of a Monooxygenase from the Gene ActVA-Orf6 of Streptomyces coelicolor Strain A3(2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9326
Spacegroup name	P 21 21 21
Unit cell lengths	47.028, 60.168, 71.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.300
Rwork	0.142
R-free	0.16800
Structure solution method	MIRAS
RMSD bond length	0.013 *
RMSD bond angle	0.990 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.059	0.136
Total number of observations	798091 *
Number of reflections	50368 *
<I/σ(I)>		12.2
Completeness [%]	99.8 *	100
Redundancy	15.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	293	ammonium sulfate, PEG 200, Tris or Hepes buffer, pH 7.0, VAPOR DIFFUSION, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.5 (M)
2	1	reservoir	HEPES	100 (mM)	pH7.0
3	1	reservoir	Tris-HCl	100 (mM)	pH8.0
4	1	reservoir	PEG200	10-15 (%)