1LPU

Low Temperature Crystal Structure of the Apo-form of the catalytic subunit of protein kinase CK2 from Zea mays

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-08-20
Detector	MACSCIENCE
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	143.190, 59.758, 45.082
Unit cell angles	90.00, 103.41, 90.00

Refinement procedure

Resolution	69.000 * - 1.860
R-factor	0.204 *
Rwork	0.204
R-free	0.24800 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1a6o
RMSD bond length	0.016
RMSD bond angle	1.438
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.000 *	1.920
High resolution limit [Å]	1.860	1.860
Rmerge	0.084 *	0.473 *
Number of reflections	31230
<I/σ(I)>	28.4	4
Completeness [%]	99.6 *	89.8
Redundancy	8.8	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.1	20 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	20 (%(w/v))
2	1	reservoir	sodium acetate	200 (mM)
3	1	reservoir	Tris-HCl	100 (mM)	pH8.1