1LPI

HEW LYSOZYME: TRP...NA CATION-PI INTERACTION

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	278
Detector technology	IMAGE PLATE
Collection date	1995-09
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	79.100, 79.100, 37.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 - 2.000
R-factor	0.17 *
R-free	0.24000 *
Starting model (for MR)	1hel
RMSD bond length	0.005
RMSD bond angle	0.020
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	SHELXL-96
Refinement software	SHELXL-96

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000	3.000
High resolution limit [Å]	2.000	2.000
Rmerge	0.096 *	0.188 *
Number of reflections	7717
<I/σ(I)>	14.08	10.01
Completeness [%]	98.5	99.09
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6		PROTEIN WAS CRYSTALLIZED FROM 2% NANO3, IN A 100 MM SODIUM ACETATE BUFFER, PH 4.6; THE PROTEIN STOCK SOLUTION CONTAINED 20 MG/ML

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1		2 (%)
3	1	1	sodium acetate	0.1 (M)