1LPB

THE 2.46 ANGSTROMS RESOLUTION STRUCTURE OF THE PANCREATIC LIPASE COLIPASE COMPLEX INHIBITED BY A C11 ALKYL PHOSPHONATE

Experimental procedure

Spacegroup name	P 42 21 2
Unit cell lengths	133.700, 133.700, 93.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.460
R-factor	0.183
Rwork	0.183
R-free	0.28500
RMSD bond length	0.013
RMSD bond angle	1.960
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.460 *	2.460 *
Rmerge	0.106 *
Total number of observations	271893 *
Number of reflections	29845 *
Completeness [%]	92.0 *	77.8 *
Redundancy	9.4 *	3.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	lipase	8 (mg/ml)
2	1	drop	colipase	2.5 (mg/ml)
3	1	drop	PEG8000	2 (%)
4	1	drop	MES	0.1 (M)
5	1	drop		0.4 (M)
6	1	drop	beta-glucoside	250 (mM)