1LPA

INTERFACIAL ACTIVATION OF THE LIPASE-PROCOLIPASE COMPLEX BY MIXED MICELLES REVEALED BY X-RAY CRYSTALLOGRAPHY

Experimental procedure

Spacegroup name	P 42 21 2
Unit cell lengths	133.400, 133.400, 92.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.040
R-factor	0.186
Rwork	0.186
RMSD bond length	0.016
RMSD bond angle	3.600
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	3.000 *
Rmerge	0.200 *
Total number of observations	97990 *
Number of reflections	15496 *
Completeness [%]	90.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	phosphatidylcholine	30 (mM)
2	1	drop	sodium taurodeoxycholate	60 (mM)
3	1	drop	panvreatic lipase	8 (mg/ml)
4	1	drop	procolipase	2.5 (mg/ml)
5	1	drop	beta-octylglucoside	250 (mM)
6	1	reservoir	MES	0.1 (M)
7	1	reservoir		0.3 (M)
8	1	reservoir	PEG8000	2 (%)