1LOY
X-ray structure of the H40A/E58A mutant of Ribonuclease T1 complexed with 3'-guanosine monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.023, 44.812, 50.105 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.400 - 1.550 |
| R-factor | 0.195 |
| Rwork | 0.189 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rga |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.400 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.059 | 0.457 |
| Number of reflections | 12848 | |
| Completeness [%] | 94.5 | 89.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 20 * | MPD,CaCl2,sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.5 (mM) | |
| 2 | 1 | drop | substrate | 3 (mM) | |
| 3 | 1 | reservoir | 2 (mM) | ||
| 4 | 1 | reservoir | sodium acetate | 50 (mM) | pH4.2 |
| 5 | 1 | reservoir | MPD | 42-52 (%) |
