1LOU
RIBOSOMAL PROTEIN S6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-23 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 52.555, 105.805, 41.204 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| Rwork | 0.197 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 2.806 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.900 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.055 | 0.314 * |
| Total number of observations | 62306 * | |
| Number of reflections | 8414 | |
| <I/σ(I)> | 18 | |
| Completeness [%] | 96.4 | 94.2 |
| Redundancy | 7.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | 0.4 (M) | ||
| 6 | 1 | reservoir | MPD | 15 (%) |
