1LOU

RIBOSOMAL PROTEIN S6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1998-10-23
Detector	MARRESEARCH
Spacegroup name	C 2 2 2
Unit cell lengths	52.555, 105.805, 41.204
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.950
Rwork	0.197
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ris
RMSD bond length	0.015 *
RMSD bond angle	2.806 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.900	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.055	0.314 *
Total number of observations	62306 *
Number of reflections	8414
<I/σ(I)>	18
Completeness [%]	96.4	94.2
Redundancy	7.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	Tris-HCl	50 (mM)
4	1	reservoir		0.2 (M)
5	1	reservoir		0.4 (M)
6	1	reservoir	MPD	15 (%)