1LOP
CYCLOPHILIN A COMPLEXED WITH SUCCINYL-ALA-PRO-ALA-P-NITROANILIDE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.330, 68.230, 40.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.800 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.700 |
| Data reduction software | WEIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.069 |
| Total number of observations | 67183 * |
| Number of reflections | 13690 |
| Completeness [%] | 79.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.5 (mM) | |
| 2 | 1 | drop | tripeptide | 6 (mM) | |
| 3 | 1 | drop | methanol | 10 (%) | |
| 4 | 1 | drop | ammonium sulfate | 38 (%sat) | |
| 5 | 1 | drop | Tris-HCl | 10 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 46 (%sat) | |
| 7 | 1 | reservoir | Tris-HCl | 10 (mM) |
