1LOF

X-RAY STRUCTURE OF A BIANTENNARY OCTASACCHARIDE-LECTIN COMPLEX AT 2.3 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	78.300, 75.400, 103.900
Unit cell angles	90.00, 92.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.19
Rwork	0.190
RMSD bond length	0.018
RMSD bond angle	3.460
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.076 *
Total number of observations	71100 *
Number of reflections	24277 *
Completeness [%]	92.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		2 (mM)
3	1	drop	OCTA	1.5 (mM)
4	1	reservoir	MPD	38 (%)
5	1	reservoir	HEPES	0.1 (mM)
6	1	reservoir		0.1 (M)