1LOE

X-RAY CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT AT 1.9 ANGSTROMS RESOLUTION OF ISOLECTIN I FROM THE SEEDS OF LATHYRUS OCHRUS

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	135.840, 63.120, 54.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.185
Rwork	0.185
RMSD bond length	0.004
RMSD bond angle	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.066 *	0.255 *
Number of reflections	27484 *	2212 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	MPD	30 (%(v/v))
3	1	drop		0.02 (M)
4	1	drop	HEPES	0.1 (M)
5	1	reservoir	HEPES	0.1 (M)