1LNW
CRYSTAL STRUCTURE OF THE MEXR REPRESSOR OF THE MEXAB-OPRM MULTIDRUG EFFLUX OPERON OF PSEUDOMONAS AERUGINOSA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.910, 72.654, 240.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.030 - 2.100 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.29400 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.140 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.045 | 0.340 |
| Total number of observations | 352292 * | |
| Number of reflections | 63205 * | |
| <I/σ(I)> | 30.7 | 3.1 |
| Completeness [%] | 90.3 | 60.5 |
| Redundancy | 5.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | CaCl2, NaCl, NaAcetate, MES, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.8 (mg/ml) | |
| 2 | 1 | drop | TCEP | 10 (mM) | |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | 65 (mM) | ||
| 5 | 1 | reservoir | MES | 6 (mM) | pH6. |
| 6 | 1 | reservoir | sodium acetate | 4 (mM) | pH5. |
| 7 | 1 | reservoir | dithiothreitol | 10 (mM) |
