1LNT
Crystal Structure of the Highly Conserved RNA Internal Loop of SRP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9196, 0.9193, 0.8985 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 35.610, 35.610, 133.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| Rwork | 0.200 |
| R-free | 0.22500 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.645 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | |
| High resolution limit [Å] | 1.600 * | |
| Rmerge | 0.049 * | 0.365 * |
| Number of reflections | 21666 * | |
| Completeness [%] | 99.9 | 99 * |
| Redundancy | 11.5 * | 5.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | MPD, Cacodylate buffer, MgCl2, CaCl2, spermidine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | Cacodylate buffer | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | CaCl2 | ||
| 5 | 1 | 1 | spermidine |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 40 (mM) | pH7.0 |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | spermine tetrahydrochloride | 12.0 (mM) | |
| 4 | 1 | drop | 80 (mM) | ||
| 5 | 1 | drop | 40 (mM) | ||
| 6 | 1 | drop | MPD | 10 (%(v/v)) | |
| 7 | 1 | reservoir | MPD | 45 (%) |
