1LNI

CRYSTAL STRUCTURE ANALYSIS OF A RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS AT ATOMIC RESOLUTION (1.0 A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1995-08-25
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 21 21 21
Unit cell lengths	64.200, 77.800, 38.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.500 - 1.000
R-factor	0.119 *
Structure solution method	STARTING MODEL WAS USED WITHOUT MR
Starting model (for MR)	1rgg
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	STARTING (MODEL WAS USED WITHOUT MR)
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500	1.020
High resolution limit [Å]	1.000	1.000
Rmerge	0.042	0.539
Number of reflections	101172 *
<I/σ(I)>	28.7	2.4
Completeness [%]	97.5	91.1
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (%)
2	1	reservoir	phosphate	0.1 (M)	pH7.2
3	1	reservoir	ammonium sulfate	25 (%sat)
4	1	reservoir	glycerol	25 (%)