1LNG
Crystal Structure of the SRP19-7S.S SRP RNA Complex of M. jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.12 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.521, 61.405, 155.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.225 * |
| Rwork | 0.224 |
| R-free | 0.27500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1D4R and 1DUL |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.700 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.099 | 0.332 |
| Total number of observations | 78892 * | |
| Number of reflections | 18606 | |
| Completeness [%] | 98.1 | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 18 * | PEG 3350, HEPES, MgAc, CaCl, DMSO, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 1 | MgAc | ||
| 4 | 1 | 1 | CaCl | ||
| 5 | 1 | 1 | DMSO |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 10 | 1 | reservoir | dimethylsulphoxide | 5 (%) | |
| 11 | 1 | reservoir | PEG3350 | 18 (%) | |
| 2 | 1 | drop | 250 (mM) | ||
| 3 | 1 | drop | beta-mercaptoethanol | 1 (%) | |
| 4 | 1 | drop | glycerol | 5 (%) | |
| 5 | 1 | drop | protein | 3 (mg/ml) | |
| 6 | 1 | drop | 5 (mM) | ||
| 7 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
| 8 | 1 | reservoir | magnesium acetate | 0.1 (M) | |
| 9 | 1 | reservoir | 0.02 (M) |
