1LMN

THE REFINED CRYSTAL STRUCTURE OF LYSOZYME FROM THE RAINBOW TROUT (ONCORHYNCHUS MYKISS)

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	76.680, 76.680, 54.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.174
RMSD bond length	0.013
RMSD bond angle	0.044
Phasing software	MERLOT
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	42.110 *
High resolution limit [Å]	1.800 *
Rmerge	0.042 *
Total number of observations	70573 *
Number of reflections	16305
Completeness [%]	91.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		277 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	acetate	0.1 (M)
2	1	reservoir	citrate	0.1 (M)
3	1	reservoir	phosphate	0.1 (M)
4	1	drop	lysozyme	18 (mg/ml)	80& of typeI and 20% of type II
5	1	drop	ammonium sulfate	30-45 (%sat)