1LM6
Crystal Structure of Peptide Deformylase from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-13 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 43 |
| Unit cell lengths | 49.709, 49.709, 91.407 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.750 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lm4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 24.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.048 * | 0.241 * |
| Number of reflections | 22277 | |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 20 * | PEG 8000, 0.2 M MgCl2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH8.0 |
