1LM6

Crystal Structure of Peptide Deformylase from Streptococcus pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-13
Detector	ADSC QUANTUM 4
Spacegroup name	P 43
Unit cell lengths	49.709, 49.709, 91.407
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 1.750
R-factor	0.208
Rwork	0.208
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lm4
RMSD bond length	0.014
RMSD bond angle	24.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	1.850
High resolution limit [Å]	1.750	1.750
Rmerge	0.048 *	0.241 *
Number of reflections	22277
<I/σ(I)>	20.6
Completeness [%]	99.5	98.1
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	20 *	PEG 8000, 0.2 M MgCl2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	PEG8000	20 (%(w/v))
3	1	reservoir		0.2 (M)
4	1	reservoir	Tris	0.1 (M)	pH8.0