1LLP
LIGNIN PEROXIDASE (ISOZYME H2) PI 4.15
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-11-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.700, 74.710, 106.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.043 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.0) |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.083 |
| Total number of observations | 243426 * |
| Number of reflections | 52732 |
| Completeness [%] | 98.3 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 15 (%sat) | |
| 3 | 1 | drop | sodium citrate | 50 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 30 (%sat) | |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) |
