1LLH
ARE CARBOXY TERMINII OF HELICES CODED BY THE LOCAL SEQUENCE OR BY TERTIARY STRUCTURE CONTACTS
Replaces: 1JOZExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | rotating-anode X-ray tube |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-02-15 |
| Detector | AREA DETECTOR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.977, 60.977, 97.386 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.000 - 1.800 |
| R-factor | 0.2 |
| Rwork | 0.192 |
| R-free | 0.24200 |
| Structure solution method | Isomorphous replacement |
| Starting model (for MR) | 2lzm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.527 |
| Data scaling software | Xengen (HOWARD, NIELSEN, XUONG) |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.440 | 1.940 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.215 | |
| Number of reflections | 17775 | |
| <I/σ(I)> | 10.76 | 1.9 |
| Completeness [%] | 81.0 | 81 |
| Redundancy | 2.37 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 1.8M Phosphate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
