1LLA

CRYSTAL STRUCTURE OF DEOXYGENATED LIMULUS POLYPHEMUS SUBUNIT II HEMOCYANIN AT 2.18 ANGSTROMS RESOLUTION: CLUES FOR A MECHANISM FOR ALLOSTERIC REGULATION

Experimental procedure

Spacegroup name	R 3 2
Unit cell lengths	117.002, 117.002, 117.002
Unit cell angles	60.02, 60.02, 60.02

Refinement procedure

Resolution	10.000 - 2.180
R-factor	0.174
Rwork	0.174
RMSD bond length	0.014
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.180 *
Rmerge	0.046 *
Total number of observations	38255 *
Completeness [%]	97.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *		taken from mangus, K.A. et al (1991). Proteins Struct. Funct. Genet., 9, 240-247. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Bis Tris	160 (mM)
2	1	reservoir	Tris-HCl	30 (mM)
3	1	reservoir	glycine	30 (mM)
4	1	reservoir	EDTA	6 (mM)
5	1	reservoir	PEG8000	4 (%)
6	1	reservoir		0.5 (M)