1LL2

Crystal Structure of Rabbit Muscle Glycogenin Complexed with UDP-glucose and Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	108
Detector technology	IMAGE PLATE
Collection date	2001-03-02
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	57.887, 106.875, 122.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.194 *
Rwork	0.194
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	1.190 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.045	0.372
Total number of observations	93616 *
Number of reflections	29472 *
<I/σ(I)>	22.1	2.7
Completeness [%]	96.7 *	88.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	4 *	8-10 mg/mL glycogenin, 0.7 to 1.2 M ammonium sulphate, and 100 mM sodium phosphate buffer pH 6.6 to 6.9, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	glycogenin	8-10 (mg/ml)
2	1	reservoir	ammonium sulfate	0.7-1.2 (M)
3	1	reservoir	sodium phosphate	100 (mM)	pH6.6-6.9