1LL1
HYDROXO BRIDGE MET FORM HEMOCYANIN FROM LIMULUS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-09 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 116.610, 116.610, 285.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.000 - 2.550 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lla |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.600 |
| Data reduction software | UCSD |
| Data scaling software | SCALA (IN CCP4) |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.300 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.188 |
| Number of reflections | 22669 | |
| <I/σ(I)> | 7.1 | 3.9 |
| Completeness [%] | 87.6 | 39.4 |
| Redundancy | 3.9 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | PROTEIN WAS CRYSTALLIZED FROM 4-6% PEG 3350, 0.2M BIS-TRIS, 0.5M NACL, 5MM H2O2. PH IN THE DROP NEAR 6.8. |
